S. Yu. Garmonov

2-Amino-4,6-dinitrophenol (2-A-4,6-DNP), or picramic acid, is widely used as an intermediate in organic synthesis, a dye in hair coloring products, an agricultural fungicide, and an explosive. Similar to other related compounds (2,4,6-trinitrophenol (2,4,6-TNF) and N-methyl-N,2,4,6-tetranitroaniline), of which it is a metabolite, 2-A-4,6-DNP is toxic to humans and animals. However, certain aspects of its detection in toxicological screening remain poorly understood. In this study, a mixture of acetone and acetonitrile in equal amounts was identified as the most effective extractant for 2-A-4,6-DNP. The application of gas chromatography–mass spectrometry (GC–MS) for detecting 2-A-4,6-DNP in the biological material extracts was analyzed and justified. The proposed method, based on the indirect determination of 2-A-4,6-DNP through O-acetyl derivative quantification, was employed to assess its stability in the biolo- gical material. 2-A-4,6-DNP was found to be detectable in the biological medium within 25 (storage temperature 18–22 °C) to 45 (storage temperature 1–3 °C) days after the contamination.

Определение биологически активных хлорнитрозамещенных бензофуроксанов методом высокоэффективной жидкостной хроматографии

The results of using the “response surface” methodology and the Box–Behnken design in the spectrophotometric determination of medicinal substances in a micellar matrix are presented on examples of dapsone and procaine. 5,7-Dichloro-4,6-dinitrobenzofuroxan is used as a reagent. The design of the neonol–water (80%)–dimethyl sulfoxide (20%) composition matrix is varied using the following factors: reagent concentration, pH, and neonol concentration. Optimization is performed using a three-level Box–Behnken plan. A significant increase in the intensity of the absorption band of the reaction products in the micellar pseudophase is shown. The optimal conditions under which medicinal substances can be determined by spectrophotometry in liquid and solid medicinal forms are found.