Feiyan Guo

Aim: To evaluate the effectiveness of a multi‐perspective ‘Baby‐Midwife‐Family’ micro‐video intervention in reducing fear of childbirth among primiparous women, while also examining its effects on childbirth self‐efficacy and depressive symptoms. Design: A non‐concurrent controlled trial. Methods: This study was conducted at a tertiary hospital in Guangzhou, China. A total of 140 eligible primiparous women were enrolled. Women admitted during Dec 2024—Jan 2025 were allocated to the historical control group ( n = 70) and received usual care, whereas those admitted during Feb—Mar 2025 were allocated to the intervention group ( n = 70) and received usual care plus the micro‐video intervention. Fear of childbirth (primary outcome), childbirth self‐efficacy, and depressive symptoms (secondary outcomes) were assessed at hospital admission, before participants entered the delivery room, and on the day after delivery. Data were analysed using repeated‐measures analysis of variance with intention‐to‐treat and per‐protocol analyses. Results: Women receiving the micro‐video intervention showed significantly greater reductions in fear of childbirth and greater improvements in childbirth self‐efficacy over time than those receiving usual care alone. Depressive symptoms decreased over time in both groups, with no significant group effect. Conclusion: The findings support the ‘Baby‐Midwife‐Family’ micro‐video intervention as a useful addition to childbirth preparation for primiparous women admitted for delivery. Impact: This brief intervention adds to routine maternity nursing care by providing timely childbirth education during the short pre‐delivery period. Reporting Method: TREND (Transparent Reporting of Evaluations with Nonrandomized Designs) Statement. Patient or Public Contribution: No patients or public contribution. Trial Registration: This study was registered with the Chinese Clinical Trial Registry (Registration No. ChiCTR2500109537, https://www.chictr.org.cn/showproj.html?proj=278878 )

Background. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real‐time polymerase chain reaction. Mouse‐derived GC‐1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. The target microRNAs (miRNAs) of lncRNAs and the target mRNAs of miRNAs were predicted by a bioinformatics analysis. Functional studies performed using the CCK‐8 assay, EdU incorporation assay, apoptosis detection, and senescence‐associated β‐galactosidase (SA‐β‐Gal) staining were conducted using GC‐1 spg cells. Results. Totals of 2,652 lncRNAs and 2,625 mRNAs were found to be differentially expressed in the testicular tissue of NOA patients when compared with patients in a control group. Dynamin 3 opposite strand (DNM3OS) was a provider of pe‐miR‐214‐5p that positively regulates miR‐214‐5p expression in GC‐1 spg cells. The E2 factor (E2F) family of transcription factor 2 (E2F2) was initially predicted and subsequently verified to be a downstream gene of miR‐214‐5p. E2F2 expression was upregulated after DNM3OS knockdown in ADR‐treated GC‐1 spg cells. Moreover, knockdown of either DNM3OS or miR‐214‐5p significantly alleviated ADR‐induced decreases in cellular activity and proliferation, as well as increases in apoptosis and senescence of mouse spermatogonial GC‐1 spg cells. Conclusions. DNM3OS was found to regulate the apoptosis and senescence of spermatogonia by providing miR‐214‐5p and decreasing E2F2 expression, suggesting it as a novel target for gene therapy of male infertility.