Maocai Li
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Maocai Li has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Feiyan Guo
Department of Obstetrics and Gynecology, Nanfang Hospital Southern Medical University Guangzhou China
1 shared publication - Qing-Jun Chu
The First School of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
1 shared publication - Qinjie Chen
Nanfang Hospital, Southern Medical University, Guangzhou, China
1 shared publication - Rui Hua
Reproductive Medicine Center, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
1 shared publication - Xuan Zhou
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China
1 shared publication - Yong-Tong Zhu
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China
1 shared publication
Latest Publications
- Open Access
- 1 January 2023
- Published by Wiley in Analytical Cellular Pathology
- Vol. 2023 (1) , 1477658
DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR‐214‐5p and Decreasing E2F2 Expression
Background. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real‐time polymerase chain reaction. Mouse‐derived GC‐1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. The target microRNAs (miRNAs) of lncRNAs and the target mRNAs of miRNAs were predicted by a bioinformatics analysis. Functional studies performed using the CCK‐8 assay, EdU incorporation assay, apoptosis detection, and senescence‐associated β‐galactosidase (SA‐β‐Gal) staining were conducted using GC‐1 spg cells. Results. Totals of 2,652 lncRNAs and 2,625 mRNAs were found to be differentially expressed in the testicular tissue of NOA patients when compared with patients in a control group. Dynamin 3 opposite strand (DNM3OS) was a provider of pe‐miR‐214‐5p that positively regulates miR‐214‐5p expression in GC‐1 spg cells. The E2 factor (E2F) family of transcription factor 2 (E2F2) was initially predicted and subsequently verified to be a downstream gene of miR‐214‐5p. E2F2 expression was upregulated after DNM3OS knockdown in ADR‐treated GC‐1 spg cells. Moreover, knockdown of either DNM3OS or miR‐214‐5p significantly alleviated ADR‐induced decreases in cellular activity and proliferation, as well as increases in apoptosis and senescence of mouse spermatogonial GC‐1 spg cells. Conclusions. DNM3OS was found to regulate the apoptosis and senescence of spermatogonia by providing miR‐214‐5p and decreasing E2F2 expression, suggesting it as a novel target for gene therapy of male infertility.
- 1 January 2023
- Published by Wiley in Analytical Cellular Pathology
- Vol. 2023 (1) , 1477658
Open AccessDNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR‐214‐5p and Decreasing E2F2 Expression
Background. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real‐time polymerase chain reaction. Mouse‐derived GC‐1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. The target microRNAs (miRNAs) of lncRNAs and the target mRNAs of miRNAs were predicted by a bioinformatics analysis. Functional studies performed using the CCK‐8 assay, EdU incorporation assay, apoptosis detection, and senescence‐associated β‐galactosidase (SA‐β‐Gal) staining were conducted using GC‐1 spg cells. Results. Totals of 2,652 lncRNAs and 2,625 mRNAs were found to be differentially expressed in the testicular tissue of NOA patients when compared with patients in a control group. Dynamin 3 opposite strand (DNM3OS) was a provider of pe‐miR‐214‐5p that positively regulates miR‐214‐5p expression in GC‐1 spg cells. The E2 factor (E2F) family of transcription factor 2 (E2F2) was initially predicted and subsequently verified to be a downstream gene of miR‐214‐5p. E2F2 expression was upregulated after DNM3OS knockdown in ADR‐treated GC‐1 spg cells. Moreover, knockdown of either DNM3OS or miR‐214‐5p significantly alleviated ADR‐induced decreases in cellular activity and proliferation, as well as increases in apoptosis and senescence of mouse spermatogonial GC‐1 spg cells. Conclusions. DNM3OS was found to regulate the apoptosis and senescence of spermatogonia by providing miR‐214‐5p and decreasing E2F2 expression, suggesting it as a novel target for gene therapy of male infertility.