Ankit Punia

The loss of biodiversity all over the world due to climate change and ecosystem destruction requires new conservation strategies. CRISPR-based gene drives offer promising opportunities for ecological restoration and biodiversity conservation, especially in aquatic environments, have a bright future. Gene drives are based on CRISPR / Cas9 technology that makes it possible to change the genetic characteristics of wild populations, which would contribute to resilience against various environmental stressors, including climate change, invasive species, and disease. CRISPR gene drives can help restore the genetic diversity of endangered species, as it allows beneficial characteristics to spread through populations and aid in ensuring the control of invasive species that endanger native ecosystems. Nevertheless, the introduction of gene drive technologies creates major ethical, ecological, and governance issues. These are the possibility of an unwanted ecological impact, problems with the transboundary flow of modified organisms, and the presence of strong regulatory frameworks to provide responsible use. This paper will examine the possible use of CRISPR-based gene drives in restoring aquatic ecosystems, in terms of their capacity to increase species adaptability, decrease extinction pressures, and recover ecosystem homeostasis. The results provide significant statistical insights, demonstrating that CRISPR-based gene drives can achieve an 85% allele frequency within just five generations and reduce invasive Asian carp populations by 60% within three generations. The regulatory and ethical factors are also addressed, and it is reasonable to note that it is important to collaborate globally and closely monitor the situation in order to reduce risks. The bright future of CRISPR-based gene drives implies new research and development to overcome the challenges and ensure that their safe and efficient use in conservation tactics.

Increased user expectations for intelligent systems fuel enhanced digitization of daily activities in library systems. We propose a new framework RT-LTM, Real-Time Library Twin Monitoring, which aims to utilize Digital Twins for real-time monitoring, predictive maintenance, and tailored service optimization in library spaces. RT-LTM framework designs a virtual model of library assets such as books, RFID-tagged materials, HVAC systems, and previous behavior analytics to achieve real-time synchronization of physical and virtual systems. The architecture integrates IoT sensors, edge-cloud computing, and AI analytics for real-time monitoring of spatial efficiency, resource consumption, and maintenance forecasting. A university library case study demonstrates the active library's real-time monitoring efficacy, resource access, energy use, and user satisfaction. The results highlight the outstanding impact of Digital Twin technology in contemporary library systems and services management.

Background: Mpox, formerly known as monkeypox, is a re-emerging viral disease. Vaccine acceptance is crucial for preventing its spread. This systematic review and meta-analysis assessed the acceptance of the Mpox vaccine among student populations. Methods: We searched electronic databases, including PubMed, Web of Science, and Embase, up to 14 September 2024. The studies included were observational, such as cross-sectional and cohort studies, and specifically assessed vaccine acceptance for Mpox vaccines among students. R version 4.4 was used to perform the meta-analysis, and sensitivity analyses were conducted to assess the robustness of the findings. The publication bias was evaluated using Doi plots. Results: Of the 143 studies initially identified, eight studies were included in the final analysis, comprising a total of 16,129 participants. The overall vaccine acceptance rate was 58.6%, with considerable variability across studies (I² = 100%). The sensitivity analyses indicated that acceptance rates ranged between 45% and 70%. The Doi plot demonstrated the presence of moderate publication bias. Conclusion: This systematic review and meta-analysis shows moderate acceptance of the Mpox vaccine among students. Future studies should investigate the factors influencing vaccine acceptance and design targeted strategies to improve coverage, which will be essential for controlling Mpox and ensuring successful vaccination campaigns.

Background: Mpox, formerly known as monkeypox, is a re-emerging viral disease. Vaccine acceptance is crucial for preventing its spread. This systematic review and meta-analysis assessed the acceptance of the Mpox vaccine among student populations. Methods: We searched electronic databases including PubMed, Web of Science, and Embase up to September 14, 2024. The studies included were observational, such as cross-sectional and cohort studies, and specifically assessed vaccine acceptance for Mpox vaccines among students. R version 4.4 was used to perform meta-analysis and sensitivity analyses were conducted to assess the robustness of the findings. Publication bias was evaluated using Doi plots. Results: Of the 143 studies initially identified, eight studies were included in the final analysis, comprising a total of 16,129 participants. The overall vaccine acceptance rate was 58.6%, with considerable variability across studies (I² = 100%). Sensitivity analyses indicated that acceptance rates ranged between 45% and 70%. The Doi plot demonstrated the presence of moderate publication bias. Conclusion: This systematic review and meta-analysis show moderate acceptance of the Mpox vaccine among students. Future studies should investigate the factors influencing vaccine acceptance and design targeted strategies to improve coverage, which will be essential for controlling Mpox and ensuring successful vaccination campaigns.

This project seeks to analyze the complex dynamics of climate change adaptation techniques in agriculture by investigating the synergistic link between diverse agricultural practices and technology intervention s via agent-based modeling. The experimental data, consisting of synthetic datasets, clarifies the iterative adaptation process by illustrating examples such as “Drought Resistant Crop-alpha” and “Water Conservation System-beta,” which demonstrate the effectiveness of swift modifications to evolving climatic circumstances. The revolutionary influence of technology adoption is shown by developments such as “Smart Irrigation-Enhanced,” which underscores its ability to improve resilience and production. The seamless integration of these methods across all agricultural phases, from initial acceptance to extensive application, highlights their interrelated and mutually reinforcing connection. Confronting difficulties, especially those associated with socio-economic dynamics and resource allocation, requires the integration of ethical and policy considerations. This study offers critical insights into the essential comprehension of climate adaptation techniques, which will inform future research and underscore the need for ethical frameworks in enhancing the efficacy of agent-based models for agricultural resilience.

This article explores the fundamental principles of the software method for calculating the gas-fired heating system of a cultivation structure, where the main source of heat is provided by ceiling-mounted gas infrared radiators. To maintain the required thermal regime within the premises, a gas air heater is utilized, designed to preheat the outdoor air to the necessary temperature before supplying it to the heated space.The calculation method is based on solving a system of interconnected equations of thermal and material balances for the cultivation structure, its enclosure, and the soil surface. Possible utilization of thermal energy from high-potential combustion products of gaseous fuel is taken into account.Using the example of the “Farmer 7.5” industrial greenhouse (Russian Federation), designed for year-round cultivation of plants in closed soil (in this case, cucumbers were the subject of study until the fruitbearing stage in the winter-spring cycle), the impacts of various factors are investigated: outside air temperature, thermal resistance of the enclosing structure on the heating system’s thermal power (in combination with the gas air heater), and variable parameters of the microclimate. The latter includes the enclosure temperature of the industrial greenhouse and the intake air temperature for general exchange ventilation needs. Specific conclusions and prospects for further scientific research are drawn based on the obtained results.