Cedric Bessire

This study investigates electrolyte jet processing of metals by plasma polishing, focusing on the polishing regimes and their governing flow dynamics, which result in different current density distributions and, consequently, different plasma polishing regimes. Experimental investigations performed at 350 V using a fixed-diameter electrolyte jet included localized current measurements and surface topography characterization, while finite element method (FEM) simulations were used to evaluate four plasma vapor–gas envelope (VGE) geometries. The experimental results revealed two distinct polishing regimes: a contact zone coinciding with the nozzle diameter, where the highest local cur-rent density reached 10.8 mA/mm² and produced a maximum material removal rate of 1.17 µm/s, and a surrounding electrolyte splash zone extending to approximately twice the nozzle diameter, where the local current density progressively decreased to 2.6 mA/mm², corresponding to a minimum material removal rate of 0.16 µm/s. Unlike previous, studies the numerical simulations showed that a constant plasma vapor–gas envelope thickness of 15.8 µm provided the closest agreement with the experimentally measured polishing profile. Based on the combined experimental and numerical results, the contact zone is interpreted as a plasma-dominated region characterized by high current density, whereas the splash zone is interpreted as a transient plasma regime in which electrochemical polishing increasingly dominates as current density decreases. This study improves the understanding of plasma electrolyte polishing (PEP), which involves both plasma-assisted and electrochemical material-removal mechanisms. The results provide guidance for im-proving surface quality and process efficiency in electrolyte-jet PEP of stainless steel.

Capillary-driven microfluidic chips have emerged as promising platforms for point-of-care diagnostics, offering portable, inexpensive, and pump-free operation. Accurate tracking of cell flow in these systems is vital for quantitative applications such as on-chip cytometry, cell counting, and biomechanical analysis. However, tracking in capillary-driven devices is challenging due to rapid cell displacements, flow instabilities, and visually similar cells. Under these conditions, conventional tracking algorithms such as TrackPy, TrackMate, SORT, and DeepSORT exhibit frequent identity switches and trajectory fragmentation. Here, we introduce FloCyT, a robust, high-speed centroid tracking tool specifically designed for capillary-driven and microfluidic flow. FloCyT leverages microchannel geometry for tracking and uses anisotropic gating for association, global flow-aware track initialisation, and channel-specific association. This enables precise tracking even under challenging conditions of capillary-driven flow. FloCyT was evaluated on 12 simulated and 4 real patient datasets using standard multi-object tracking metrics, including IDF1 and MOTA, ID switches, and the percentage of mostly tracked objects. The results demonstrate that FloCyT outperforms both standard and flow-aware-modified versions of TrackPy and SORT, achieving higher accuracy, more complete trajectories, and fewer identity switches. By enabling accurate and automated cell tracking in capillary-driven microfluidic devices, FloCyT enhances the quantitative sensing capability of image-based microfluidic diagnostics, supporting novel, low-cost, and portable cytometry applications.

The advent of the additive manufacturing of Ti-6Al-4V (Ti64) alloys has facilitated the production of complex geometries for various industrial applications. Nevertheless, the inherent surface roughness of selective laser melting (SLM)-produced parts remains a critical limitation, adversely affecting fatigue life, wear, corrosion, and compliance with stringent surface quality standards, for example those required in hygienic applications. Conventional post-processing methodologies, encompassing grinding and electropolishing, are frequently multi-stage, labor-intensive, and reliant on hazardous electrolytes, which thus limits their use for certain applications. In this study, plasma electrolytic polishing (PEP) was evaluated as a single-step finishing process for 3D-printed Ti64 components. The findings indicate that PEP efficiently diminished surface roughness from initial values of approximately 9–10 µm to as low as 0.38–0.5 µm within a time frame of 15–20 min, depending on the initial surface condition. These outcomes meet hygienic surface requirements while ensuring the use of environmentally compatible electrolytes. The findings establish PEP as a non-mechanical, efficient, and scalable additive-manufacturing post-processing strategy. It has the capacity to supersede conventional multi-stage workflows and offer substantial reductions in cost, time, and environmental impact.

Blood tests are widely used in modern medicine to diagnose certain illnesses and evaluate the overall health of a patient. To enable testing in resource-limited areas, there has been increasing interest in point-of-care (PoC) testing devices. To process blood samples, liquid mixing with active pumps is usually required, making PoC blood testing expensive and bulky. We explored the possibility of processing approximately 2 μL of whole blood for image flow cytometry using capillary structures that allowed test times of a few minutes without active pumps. Capillary pump structures with five different pillar shapes were simulated using Ansys Fluent to determine which resulted in the fastest whole blood uptake. The simulation results showed a strong influence of the capillary pump pillar shape on the chip filling time. Long and thin structures with a high aspect ratio exhibited faster filling times. Microfluidic chips using the simulated pump design with the most efficient blood uptake were fabricated with polydimethylsiloxane (PDMS) and polyethylene oxide (PEO). The chip filling times were tested with 2 μL of both water and whole blood, resulting in uptake times of 24 s for water and 111 s for blood. The simulated blood plasma results deviated from the experimental filling times by about 35% without accounting for any cell-induced effects. By comparing the flow speed induced by different pump pillar geometries, this study offers insights for the design and optimization of passive microfluidic devices for inhomogenous liquids such as whole blood in sensing applications.