Michael Sandino C. Flores
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Michael Sandino C. Flores has a total of 9 co-authors. Here are the top co-authors by number of shared publications:
- Divina M. Amalin
Department of Biology, De La Salle University, Manila 0922, Philippines
1 shared publication - Evangelyn C. Alocilja
Nano-Biosensors Lab, Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA
1 shared publication - Florinia E. Merca
Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Baños, College, Los Baños 4031, Philippines
1 shared publication - Lilia M. Fernando
Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Baños, College, Los Baños 4031, Philippines
1 shared publication - Ma. Anita Mascarenas Bautista
Functional Genomics Laboratory, National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City 1101, Philippines
1 shared publication - Ma. Carmina C. Manuel
Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Los Baños 4031, Philippines
1 shared publication
Latest Publications
- Open Access
- 24 January 2025
- Published by MDPI AG in Biosensors
- Vol. 15 (2) , 71
DNA-Based Nanobiosensor for the Colorimetric Detection of Dengue Virus Serotype 2 Synthetic Target Oligonucleotide
Annually, the Philippines is burdened by a high number of infections and deaths due to Dengue. This disease is caused by the Dengue virus (DENV) and is transmitted from one human host to another by the female Aedes aegypti mosquito. Being a developing country, most of the high-risk areas in the Philippines are resource-limited and cannot afford equipment for detection and monitoring. Moreover, traditional clinical diagnoses of DENV infection are costly and time-consuming and require expertise. Hence, it is important to establish effective vector control and surveillance measures. In this study, we developed a DNA-based nanobiosensor for the colorimetric detection of Dengue virus serotype 2 (DENV-2) synthetic target DNA (stDNA S2) using gold nanoparticles (AuNPs). We successfully functionalized dextrin-capped gold nanoparticles with the designed DENV-2 oligonucleotide probes. The detection of the complementary stDNA S2, indicated by the pink-colored solution, was successfully performed within 15 min using 0.40 M NaCl solution. We were able to detect up to 36.14 ng/μL of stDNA S2 with some cross-reactivity observed with one non-complementary target. We believe that our study offers a basis for developing nanobiosensors for other DENV serotypes.
- 24 January 2025
- Published by MDPI AG in Biosensors
- Vol. 15 (2) , 71
Open AccessDNA-Based Nanobiosensor for the Colorimetric Detection of Dengue Virus Serotype 2 Synthetic Target Oligonucleotide
Annually, the Philippines is burdened by a high number of infections and deaths due to Dengue. This disease is caused by the Dengue virus (DENV) and is transmitted from one human host to another by the female Aedes aegypti mosquito. Being a developing country, most of the high-risk areas in the Philippines are resource-limited and cannot afford equipment for detection and monitoring. Moreover, traditional clinical diagnoses of DENV infection are costly and time-consuming and require expertise. Hence, it is important to establish effective vector control and surveillance measures. In this study, we developed a DNA-based nanobiosensor for the colorimetric detection of Dengue virus serotype 2 (DENV-2) synthetic target DNA (stDNA S2) using gold nanoparticles (AuNPs). We successfully functionalized dextrin-capped gold nanoparticles with the designed DENV-2 oligonucleotide probes. The detection of the complementary stDNA S2, indicated by the pink-colored solution, was successfully performed within 15 min using 0.40 M NaCl solution. We were able to detect up to 36.14 ng/μL of stDNA S2 with some cross-reactivity observed with one non-complementary target. We believe that our study offers a basis for developing nanobiosensors for other DENV serotypes.