Kossi Kabo

Escherichia ruysiae is a recently characterized species within the Escherichia genus, often misclassified as E. coli due to limitations in existing operating procedures and diagnostic databases. In this study, we report the first isolation and genomic characterization of E. ruysiae in Togo, from a five-year-old female patient who was hospitalized with gastroenteritis and bloody diarrhea and subsequently died after eight days. Biochemical tests and MALDI-TOF initially identified the microorganism as E. coli, but phylogenomic and Average Nucleotide Identity (ANI) analysis confirmed it to be E. ruysiae, Clade IV with enteroaggregative associated genes. Whole genome sequencing of the strain FK53-34 enables the identification of resistance genes, including bla_EC-15, eptA, and pmrF. The virulence profile of the strain included, but was not limited to aap, aatABC, and senB genes, which may support its pathogenicity and virulence. Multilocus sequence typing (MLST) did not match any known sequence type, which is obvious for a newly characterized microorganism. This study highlights the critical need for enhanced diagnostic tools and surveillance systems to identify emerging pathogens, including Escherichia ruysiae.

Escherichia ruysiae is a recently characterized species within the Escherichia genus, often misclassified as E. coli due to limitations in existing operating procedure and diagnostic databases. In this study, we report the first isolation and genomic characterization of E. ruysiae in Togo, from a five-year-old female patient who was hospitalized with gastro-enteritis and bloody diarrhea and subsequently died after eight days. Biochemical tests and MALDI-TOF initially identified the microorganism as E. coli, but phylogenomic and Average Nucleotide Identity (ANI) analysis confirmed it to be E. ruysiae, Clade IV with enteroaggregative associated genes. Whole genome sequencing of the strain FK53-34 enables the identification of resistance genes including blaPBP, blaampH, acrA, emrF, baeS et tolC and various aminoglycoside-modifying enzymes, blaEC-15, eptA and pmrF. The virulence profile of the strain included but not limited to, aap, aatABC, senB, prmF genes which support its pathogenicity and virulence. Multilocus sequence typing (MLST) did not match any known sequence type, which is obvious for a newly characterized mi-croorganism. This study highlights the critical need for enhanced diagnostic tools and surveillance systems to identify emerging pathogens, including Escherichia ruysiae

Achieving sustainability goals in developing countries is crucial because of vulnerabilities related to climate change, capital scarcity and increased investment risks. Entrepreneurship is a means of overcoming the ecological, economic and social problems and challenges in terms of transport, consumption, production, development and food that the continent is facing as a result of the remarkable growth in its urban population. Innovative young companies are proposing solutions that take into account the cultural, social and economic realities of users. This potential for businesses to have an impact on sustainability is limited by the financial difficulties associated with the traditional business development model. Using an approach centred on life in the region, this article highlights the opportunities represented by innovative entrepreneurship in territories in need of sustainability. It aims to stimulate reflection on the need to rethink the economic model for innovative businesses with a sustainable impact, whose solutions need to be widely disseminated by considering other forms of investment. It is therefore suggested that local administrators, be they municipalities, cities or regions, should themselves become entrepreneurs by investing in impact businesses. This article invites to consider territorial entrepreneurship as consider entrepreneurship as a dynamic for anchoring sustainable innovations in regions.