Toon Van Cauteren

Purpose: To investigate the contrast media iodine dose dependency of radiation‐induced DNA double‐strand breaks (DSBs) during a coronary computed tomography angiography (CCTA) scan.Methods: This prospective patient study was approved by the ethical committee. Between November 2018 and July 2019, 50 patients (31 males and 19 females, mean age 64 years) were included in the study, 45 CCTA and five noncontrast‐enhanced (NCE) cardiac computed tomography (CT) patients. A single‐heartbeat scan protocol with a patient‐tailored contrast media injection protocol was used, administering a patient‐specific iodine dose. DNA double‐strand breaks were quantified using a γH2AX foci assay on peripheral blood lymphocytes. The net amount of γH2AX/cell was normalized to the individual patient CT dose by the size‐specific dose estimate (SSDE). Correlation between the administered and blood‐iodine dose and the SSDE normalized amount of DNA DSBs was investigated using a Pearson correlation test.Results: CCTA patients were scanned with a mean CTDI_vol of 10.6 ± 5.6 mGy, corresponding to a mean SSDE of 11.3 ± 5.3 mGy while the NCE cardiac CT patients were scanned with a mean CTDI_vol of 6.00 ± 1.8 mGy, corresponding to a mean SSDE of 6.6 ± 2.7 mGy. The administered iodine dose ranged from 16.5 to 34.0 gI in the CCTA patients, resulting in a blood‐iodine dose range from 5.1 to 15.0 gI in the exposed blood volume. A significant linear relationship (r = 0.79, p‐value < 0.001) was observed between the blood iodine dose and SSDE normalized radiation‐induced DNA DSBs. A similar significant linear relationship (r = 0.62, p‐value < 0.001) was observed between the administered iodine dose and SSDE normalized radiation‐induced DNA DSBs.Conclusions: This study shows that contrast media iodine dose increases the level of radiation‐induced DNA DSBs in peripheral blood lymphocytes in a linear dose‐dependent manner with CCTA. Importantly, the level of DNA DSBs can be reduced by lowering the administered iodine dose.

OBJECTIVE. The purpose of this study is to investigate the contributing effect of contrast media (CM) iodine dose on radiation-induced DNA damage in blood lymphocytes during a cardiac CT scan. MATERIALS AND METHODS. The minipigs were exposed 12 times in total to a fixed cardiac CT scan protocol. An unenhanced and two CM injection protocols were considered, the latter with 50% saline diluted (160 mg I/mL) and standard iodixanol. Blood samples were collected before and after CT, and radiation-induced DNA double-strand breaks were assessed using γ-H2AX (H2A histone family member X) immunofluorescent staining of the blood lymphocytes. Significant differences in foci numbers were investigated with an independent sample t test. In addition, a numeric dosimetry model was applied that simulates the cardiac CT scan, with the heart represented by a blood volume containing a mixture of six iodine concentrations (0, 10, 20, 30, 40, and 50 mg I/mL). RESULTS. Compared with the unenhanced (0 mg I/mL) protocol, the numbe...

The study objective is to investigate the impact of a wide range of contrast media (CM) iodine concentrations on CT enhancement at constant total iodine dose (TID) and iodine delivery rate (IDR). Seven injection protocols, based on different iodine concentrations ranging from 120 to 370 mg I/mL, were assessed on 4 minipigs at a constant TID of 320 mg I/kg and IDR of 0.64 g I/s. Dynamic images were acquired on a clinical 64-slice MDCT scanner for 120 s with the abdominal aorta, vena cava inferior and liver parenchyma in the field-of-view. Maximal enhancement, time-to-peak and peak width were assessed. The enhancement curve characteristics were correlated with CM iodine concentration. In particular, CM with lower iodine concentrations yielded a significant increased maximal enhancement and peak width compared to the standard-of-care concentrations: e.g. in the aorta, 245 HU maximal enhancement and 9.2 s peak width with the 320 mg I/mL iodine concentration increased to 291 HU and 16.1 s with 160 mg I/mL. When maintaining a constant TID and IDR, by compensating injection rate and volume, injection of a CM with reduced iodine concentration results in a diagnostically beneficial higher maximal enhancement and longer enhancement peak duration.

Current typical fetal MR protocols with 3-T magnets with imaging time limited to less than 30 minutes are associated with a cumulative temperature increase of less than 1°C in fetal organs of miniature pigs, although with high-SAR regimens that last more than 1 hour, a maximum increase of 2.5°C can be reached. PurposeTo determine the changes in temperature within the gravid miniature pig uterus during magnetic resonance (MR) imaging at 3 T.Materials and MethodsThe study received ethics committee approval for animal experimentation. Fiber-optic temperature sensors were inserted into the fetal brain, abdomen, bladder, and amniotic fluid of miniature pigs (second trimester, n = 2; third trimester, n = 2). In the first trimester (n = 2), the sensors were inserted only into the amniotic fluid (three sacs per miniature pig, for a total of six sacs). Imaging was performed with a 3-T MR imager by using different imaging protocols in a random order for animal, each lasting approximately 15 minutes. The first regimen consisted of common sequences used for human fetal MR examination, including normal specific absorption rate (SAR). The second regimen consisted of five low-SAR sequences, for which three gradient-echo sequences were interspersed with two diffusion-weighted imaging series. Finally, a high-SAR regimen maximiz...

Objectives To assess image quality in abdominal CT at low tube voltage combined with two types of iterative reconstruction (IR) at four reduced contrast agent dose levels. Methods Minipigs were scanned with standard 320 mg I/mL contrast concentration at 120 kVp, and with reduced formulations of 120, 170, 220 and 270 mg I/mL at 80 kVp with IR. Image quality was assessed by CT value, dose normalized contrast and signal to noise ratio (CNRD and SNRD) in the arterial and venous phases. Qualitative analysis was included by expert reading. Results Protocols with 170 mg I/mL or higher showed equal or superior CT values: aorta (278–468 HU versus 314 HU); portal vein (205–273 HU versus 208 HU); liver parenchyma (122–146 HU versus 115 HU). In the aorta, all 170 mg I/mL protocols or higher yielded equal or superior CNRD (15.0–28.0 versus 13.7). In liver parenchyma, all study protocols resulted in higher SNRDs. Radiation dose could be reduced from standard CTDI_vol = 7.8 mGy (6.2 mSv) to 7.6 mGy (5.2 mSv) with 170 mg I/mL. Conclusion Combining 80 kVp with IR allows at least a 47 % contrast agent dose reduction and 16 % radiation dose reduction for images of comparable quality. Key Points • There is a balance between image quality, contrast dose and radiation dose. • Iterative reconstruction has a major, positive impact on this balance. • Both contrast dose and radiation dose can be reduced in abdominal CT. • The trade-off can be quantitatively described by a 3D model. • Contrast and radiation dose can be tailored according to specific safety concerns.