Akhat B. Bakirov

Introduction. The main cause of the able-bodied population’s death in the Russian Federation is cardiovascular diseases. It is known that one of the most important factors of cardiovascular risk is a violation of lipid metabolism, which is an indicator of atherogenesis processes activation. In this regard, it is relevant to study the lipid spectrum and its effect on the cardiovascular risk’s level for workers of various industries, who are exposed to a complex of harmful production factors. The study aims to investigate lipid metabolism and atherosclerotic processes in chemical production workers for the opportune implementation of therapeutic and preventive measures. Materials and methods of research. Comprehensive clinical and hygienic studies in the chemical industry have been carried out. Hygienic studies and assessment of working conditions of employees were accomplished using the current regulatory documents. Surveyed were divided into 3 groups: machine operators (I group), machinery repair locksmiths (II group), control and measuring devices’ locksmiths (CMD) (III group - comparison). To identify risk factors for cardiovascular diseases (CVD), a screening examination was conducted within the framework of periodic medical examinations of 3761 male workers, a study of the lipid spectrum in 846 workers. Employees with high and very high cardiovascular risk according to the SCORE system underwent in-depth medical examination using clinical laboratory, functional, ultrasound examination methods (179 people). The relative risk and etiological proportion were calculated in order to establish a causal relationship between the complex of harmful production factors and the formation of hypercholesterolemia. Results. The data of the conducted studies showed that the factors of cardiovascular risk in workers of I and II groups have harmful working conditions on the workplace (general class of working conditions 3.2), smoking, obesity, hypertension, which are more common in the group of machinery repairmen which was compared with workers of other professions. It was found that the average values of total cholesterol in blood serum, LDL cholesterol were higher in the examined I-II groups than III group. Analysis of the state of the cardiovascular system main indicators for workers of various professions obtained as a result of in-depth examination in a hospital revealed, that signs carotid arteries atherosclerotic changes were more often registered in workers of I-II groups. The revealed indicators of dyslipidemia and atherosclerosis increased in proportion to the length of service in harmful working conditions. Conclusion. The conducted research allowed to substantiate the algorithm of stage-by-stage examination, dynamic monitoring of the chemical production workers’ health’s state in order to detect atherosclerosis earlier and integrate therapeutic and preventive measures timely.

The aim of the study was to study the effect of hepatoprotective drugs on the expression of the Sod1 gene in rats with ethanol liver damage.Materials and methods. Male outbred white rats were used in the experiment. Five groups of animals were formed, 14 individuals each. Distilled water was administered to rats of the 1st group (control); Group 2 — ethanol at a dose of 5 g/kg of body weight; Group 3 — ethanol and heptor at a dose of 72 mg/kg; Group 4 — ethanol and mexidol at a dose of 50 mg/kg; Group 5 — ethanol and OMU at a dose of 50 mg/kg. The drugs were administered 1 hour before the introduction of ethanol. 24 and 72 hours after the introduction of ethanol (7 individuals), the animals were decapitated and the liver was removed. The expression level of the Sod1 gene was assessed using real-time reverse transcription PCR.Results. The fold change in Sod1 expression in rat liver after 24 h practically did not change in response to the introduction of ethanol to the animals. A tendency to a slight decrease was observed in relation to changes in the expression of Sod1 with the use of heptor and mexidol, while under the influence of OMU, the expression level increased moderately. After 72 h, the exposure to ethanol was accompanied by a slight decrease in the frequency of expression of the Sod1 gene. A similar trend was observed with respect to changes in Sod1 expression with the use of heptor, mexidol, and OMU.Conclusion. The results obtained indicate that the introduction of both ethanol and the prophylactic use of hepatoprotective drugs did not lead to significant changes in the level of Sod1 gene expression in rat liver. Additional studies are needed to identify the mechanisms of regulation of the antioxidant system, as well as the search for drugs that affect the transcriptional activity of genes.

Introduction. In Russia and abroad, there has been a clear trend towards an increase in cases of sudden death in the workplace from circulatory system diseases over the past decade. Materials and methods. During the study, the databases of the State Labor Inspectorate in the Republic of Bashkortostan for 2014-2018 were studied, including accident investigation reports, information on a special assessment of working conditions, medical examinations, forensic medical examinations, and other materials. Results. The leading cause of sudden death in the workplace in more than 90% of cases were circulatory system diseases (CSD). The maximum number of cases of sudden death from CSD was recorded in the age group of 56-60 years. Working condit ions represented the overwhelming majority of the deceased - 82.8%. In 13.7% of cases, they were drivers of transport. The vast majority of deaths occurred in the morning (from 6 to 12) and daytime (from 12 to 18). The working conditions of workers from deceased CSD in most cases (59.8%) corresponded to the permissible class (class 2); under harmful conditions (subclass 3.1-3.3), 24.9% worked. Most often, some form of acute coronary heart disease (CHD) was mentioned as the immediate cause of death (64.0%). Among acute forms of coronary heart disease in medical documents, in more than 50% of cases, code I 24.8 “Other forms of acute coronary heart disease” was established. “Acute myocardial infarction” was verified in 11.1% of patients and was the leading cause of death in men. In the group “Cerebrovascular Diseases,” “Intracerebral hemorrhage” was most often diagnosed. Conclusion. The obtained results substantiate the need to develop measures to prevent sudden death in the workplace, aimed primarily at preventing the development, progression, and early diagnosis of diseases of the circulatory system.

Objective: The development of asthma and its related phenotypes is most likely due to the polymorphism of the so-called modifier genes. The goal of this study was to evaluate the polymorphic locus rs1695 of the GSTP1 gene association with risk factors for developing asthma and its phenotypic manifestations. Methods: This case-control study involved 164 patients with confirmed asthma diagnosis and 147 age- and sex-matched controls. Patients were divided into two groups: with (n = 121) and without complications (n = 43). Among asthmatic patients, 34 manifested hypersensitivity to household allergens. The GSTP1 rs1695 polymorphism was genotyped using the technique of polymerase chain reaction-restriction fragment length polymorphism. Results: There were no differences between patients and controls in allelic or genotype frequencies of polymorphic locus rs1695 of the GSTP1 gene. However, the frequency of the A/A genotype in the patient group with complications was significantly lower than that in complication-free patients (p = 0.040), while the frequency of the G allele was higher in patients with complications (p = 0.030). The frequency of the A/A genotype was decreased in the patient group with an allergic reaction to household allergens in comparison with controls (p = 0.037). Conclusion: These results suggest that the carriage of the A/A genotype of polymorphic locus rs1695 of the GSTP1 gene is a protective factor in the development of complications and an allergic reaction to house allergens among asthmatics, while the carriage of the G allele is associated with an increased risk for asthma complications.

Introduction. Agricultural crops grown on garden plots are mainly used for personal consumption, are not subject to safety control, and therefore, the chemical composition of the produced crop products may significantly affect the accumulation or deficiency of chemical elements in the human body. Material and methods. To assess the chemical composition of the main products of plant origin produced in the regions of the Republic of Bashkortostan with different economic specialization, the content of 9 trace elements in carrots, table beets and potatoes was determined by atomic absorption spectrometry (n = 353). Using the total coefficient of anthropogenic load (Ka.n. = ∑Ci/PDUi), the territory of the Republic was ranked according to the degree of contamination of vegetable crops with toxic elements (lead, cadmium). The influence of trace elements contained in crop products on the health of the region’s population was studied using the risk assessment method. Results. Vegetables with a high content of cadmium (10.8% of the analyzed samples) occupy the largest share in the total volume of crop production that does not meet hygiene standards. When ranking the territory of the Republic on the basis of health risk assessment and the degree of contamination of vegetable crops with toxic elements, territories with a developed mining and petrochemical industry were found to be most unfavorable zones. Conclusion. The content of chemical elements in vegetable crops produced on household plots varies in a wide range, depending on the type of plant products and the place of growth. The lack of standards for the permissible content of certain trace elements in food raw materials and food products, which are priority pollutants in a number of regions, and safety control of products produced in private farms increase the risk of consumption of contaminated products by the population.

Introduction. The high prevalence of diseases of the circulatory system of workers in various sectors of the economy, the lack of data on the effects of harmful production factors on the formation of cardiovascular diseases in petrochemical industry workers justify the importance of conducting special studies on these petrochemical complex plants with the aim of assessing cardiovascular risk with the subsequent development of preventive measures. Material and methods. The objects of the study were workers in the production of monomers (ethylene, propylene, ethylbenzene, isoprene, divinyl) of petrochemical enterprises. Hygienic studies of the working conditions of workers included an assessment of the factors of the working environment and the labor process using generally accepted methods. Comprehensive clinical examination of workers included periodic examinations (n = 2634) with calculation of total and relative cardiovascular risk using the SCORE system, using questionnaire data, a Reeder L questionnaire and an in-depth clinical diagnostic medical examination (n = 101) with the definition total cardiovascular risk. Results. The most significant occupational factors of the labor process for workers in the main profession - panmen were established to include the combination of a chemical factor with the occupational noise and labor intensity, with an overall assessment of working conditions 3.2. The class of working conditions of workers of the comparison group (locksmith in instrumentation and automation corresponds to the permissible). There were revealed a high prevalence of arterial hypertension and other factors of cardiovascular risk. A higher dependence of the arterial hypertension level on age and length of service in the group of panmen was established when compared to the mechanicians of instrumentation and automation. Using mathematical models of multiple regression, the likely dynamics of the level of arterial hypertension and the left ventricular myocardial mass index were calculated under the influence of increasing age and other risk predictors. Conclusion. The results of a clinical study showed workers with severe arterial hypertension, a high and very high risk of death from cardiovascular disease on the SCORE scale, a high and very high overall cardiovascular risk, signs of dyslipidemia, obesity, high stress levels to require close attention. It is important to timely diagnose lesions of target organs, to conduct rational prevention aimed at reducing risk factors. According to the results of the study, there were developed preventive measures aimed at reducing cardiovascular morbidity and cardiovascular risk, prolonging the longevity of workers in petrochemical industries.

Introduction. The high prevalence of diseases of the circulatory system of workers in various sectors of the economy, the lack of data on the effects of harmful production factors on the formation of cardiovascular diseases in petrochemical industry workers justify the importance of conducting special studies on these petrochemical complex plants with the aim of assessing cardiovascular risk with the subsequent development of preventive measures. Material and methods. The objects of the study were workers in the production of monomers (ethylene, propylene, ethylbenzene, isoprene, divinyl) of petrochemical enterprises. Hygienic studies of the working conditions of workers included an assessment of the factors of the working environment and the labor process using generally accepted methods. Comprehensive clinical examination of workers included periodic examinations (n = 2634) with calculation of total and relative cardiovascular risk using the SCORE system, using questionnaire data, a Reeder L questionnaire and an in-depth clinical diagnostic medical examination (n = 101) with the definition total cardiovascular risk. Results. The most significant occupational factors of the labor process for workers in the main profession - panmen were established to include the combination of a chemical factor with the occupational noise and labor intensity, with an overall assessment of working conditions 3.2. The class of working conditions of workers of the comparison group (locksmith in instrumentation and automation corresponds to the permissible). There were revealed a high prevalence of arterial hypertension and other factors of cardiovascular risk. A higher dependence of the arterial hypertension level on age and length of service in the group of panmen was established when compared to the mechanicians of instrumentation and automation. Using mathematical models of multiple regression, the likely dynamics of the level of arterial hypertension and the left ventricular myocardial mass index were calculated under the influence of increasing age and other risk predictors. Conclusion. The results of a clinical study showed workers with severe arterial hypertension, a high and very high risk of death from cardiovascular disease on the SCORE scale, a high and very high overall cardiovascular risk, signs of dyslipidemia, obesity, high stress levels to require close attention. It is important to timely diagnose lesions of target organs, to conduct rational prevention aimed at reducing risk factors. According to the results of the study, there were developed preventive measures aimed at reducing cardiovascular morbidity and cardiovascular risk, prolonging the longevity of workers in petrochemical industries.

The problem of the studies on occupational cancer diseases is one of the most important issues of occupational health. Its significance is due to a gap of knowledge, an increase in morbidity and mortality rate from cancer. The Republic of Bashkortostan is one of the most developed industrial regions of Russia with a significant number of production processes where carcinogenic substances and factors are used. The analysis has shown that the greatest number of carcinogens is found in the production of continuous fiberglass, rubber products, lubricants and cooling materials, fuels, and organic substances. Over the past 10 years, only 11 cases of work-related malignancies have been identified in the republic. The main reasons for the development of occupational neoplasms (skin, larynx, lungs, blood) are Group I chemicals (the degree of evidence is sufficient) (72.7 %). Exposure to physical factors (ionizing radiation) led to the development of occupational cancer (kidney, thyroid, connective tissue) in 27.3 % of patients. A low identification rate of occupational neoplasms in the republic indicates the need to be increasingly cautious about cancer at the stages of preventive health check-ups of workers exposed to carcinogenic factors and substances.

Introduction. Over the past decade, in Russia and abroad, there has been a clear trend towards an increase in cases of sudden death in the workplace from the disease of the circulatory system. Material and methods. During the study, there were studied the databases of the State Labor Inspectorate in the Republic of Bashkortostan in 2014-2018, including accident investigation reports, information on a special assessment of working conditions, medical examinations, forensic medical examinations and other materials. Results. Diseases of the circulatory system (DSC) were the main cause of sudden death in the workplace in more than 90% of cases. The maximum number of cases of sudden due to DSC was recorded in the age group of 56-60 years. The overwhelming majority of the deceased cases were represented by working occupations - 82.8%, drivers of transport were in 13.7% of cases. The vast majority of deaths occurred in the morning (from 6 to 12) and daytime (from 12 to 18) hours. The working conditions of deceased DCS workers in most cases (59.8%) corresponded to the permissible class (class 2); under harmful conditions (subclass 3.1 - 3.3) 24.9% cases worked. Most often, some form of acute coronary heart disease (CHD) was mentioned as the immediate cause of death (64.0%). Among acute forms of coronary heart disease in medical documents in more than 50% of cases the code I 24.8 “Other forms of acute coronary heart disease” was established. “Acute myocardial infarction” was verified in 11.1% of cases and was the main cause of death in men. In the group “Cerebrovascular Diseases”, “Intracerebral hemorrhage” was most often diagnosed. Conclusion. The obtained results substantiate the need to develop measures to prevent sudden death in the workplace, aimed primarily at preventing the development, progression and early diagnosis of DSC.

Introduction. Over the past decade, in Russia and abroad, there has been a clear trend towards an increase in cases of sudden death in the workplace from the disease of the circulatory system. Material and methods. During the study, there were studied the databases of the State Labor Inspectorate in the Republic of Bashkortostan in 2014-2018, including accident investigation reports, information on a special assessment of working conditions, medical examinations, forensic medical examinations and other materials. Results. Diseases of the circulatory system (DSC) were the main cause of sudden death in the workplace in more than 90% of cases. The maximum number of cases of sudden due to DSC was recorded in the age group of 56-60 years. The overwhelming majority of the deceased cases were represented by working occupations - 82.8%, drivers of transport were in 13.7% of cases. The vast majority of deaths occurred in the morning (from 6 to 12) and daytime (from 12 to 18) hours. The working conditions of deceased DCS workers in most cases (59.8%) corresponded to the permissible class (class 2); under harmful conditions (subclass 3.1 - 3.3) 24.9% cases worked. Most often, some form of acute coronary heart disease (CHD) was mentioned as the immediate cause of death (64.0%). Among acute forms of coronary heart disease in medical documents in more than 50% of cases the code I 24.8 “Other forms of acute coronary heart disease” was established. “Acute myocardial infarction” was verified in 11.1% of cases and was the main cause of death in men. In the group “Cerebrovascular Diseases”, “Intracerebral hemorrhage” was most often diagnosed. Conclusion. The obtained results substantiate the need to develop measures to prevent sudden death in the workplace, aimed primarily at preventing the development, progression and early diagnosis of DSC.